The advantage of this model is that it closely mimics the natural history of human type 2 diabetes without leptin receptor deficiency, as in db/db mice, and leptin deficiency, as in ob/ob mice. Initially, normal mice are fed with high fat diet to induce obesity, which results in hyperinsulinemia and insulin resistance. To induce hyperglycemia, a low dose of streptozotocin is administered to induce moderate destruction of pancreatic beta cells, which results in low insulin secretion. The combination of severe insulin resistance and hyperglycemia with intact leptin signaling makes this an ideal model to test the efficacy of anti-diabetic compounds.
Triangulum scientists have many years of extensive experience in this model, and have successfully tested several anti-diabetic compounds and obtained reproducible results. We offer preclinical efficacy, proof-of-concept, and mechanism of action studies with your drug candidates in this model. We work with our clients in providing scientific guidance, customized study design, end-point analysis, histopathology services, and will assist you with professional interpretation of the data. Please contact us with your specific needs.
Relevant publications by our scientists: 1. Watts LM, Manchem VP, Leedom TA, Rivard AL, McKay RA, Bao D, NeroladakisT, Monia BP, Bodenmiller DM, Cao JX, Zhang HY, Cox AL, Jacobs SJ, Michael MD, Sloop KW, Bhanot S. Diabetes. 2005, 54(6):1846-1853. PMID: 15919808