Hyperinsulinemic-Euglycemic clamp methodology is considered the "Gold Standard" in assessing insulin sensitivity and glucose utilization in animal models and in humans. In this model, insulin is infused at a constant rate to create hyperinsulinemia, and glucose is infused at a variable rate to maintain plasma glucose at a steady state. The rate of glucose infusion (GINF) is an indicator of insulin sensitivity. GINF represents the mass flux of glucose necessary to counter the total effect of insulin to deplete the extracellular glucose pool, which is a sensitive index of whole body insulin sensitivity. In addition, this powerful methodology allows one to investigate the activity of compounds on hepatic glucose production and tissue specific glucose uptake by tracer glucose infusions.

Triangulum scientists have several years of experience in successfully demonstrating the effect of investigative compounds in hyperinsulinemic-euglycemic clamp method. We are pleased to offer this service to our clients and assist with your projects that require this methodology. Please contact us for more details.

Relevant publications by our scientists: 1. Yu XX, Watts LM, Manchem VP, Chakravarty K, Monia BP, McCaleb ML, Bhanot S. PLoS One, 2013 Jul 29;8(7):e66923. doi: 10.1371/journal.pone.0066923. PMID: 23922646

2. Zemany L, Bhanot S, Peroni OD, Murray SF, Moraes-Vieira PM, Castoldi A, Manchem P, Guo S, Monia BP, Kahn BB. Diabetes. 2015 May;64(5):1603-14. Doi: 10.2337/db14-0970. PMID: 2552491