UUO is the most widely used model to study renal fibrosis. This model is made by a surgical ligation of the ureter. The obstruction of the urinary pathway causes kidney enlargement and progressive damage of renal structures which leads to chronic renal dysfunction and rapid tubular atrophy. This triggers the inflammatory process inducing extracellular matrix (ECM) deposition and interstitial fibrosis development. These pathological manifestations caused by the obstruction are established within 7 days in mice. Triangulum Biopharma offers UUO model in mice for preclinical efficacy, proof-of-concept and mechanism of action studies. In addition, Triangulum Biopharma also conducts comprehensive clinical chemistry for the plasma/serum bio-markers, hematocrit, plasma erythropoietin, ferritin measurements, cytokine analysis, biochemical and ELISA assays, gene expression analysis by qPCR, renal hydroxyproline assays and comprehensive histopathology evaluation.
UUO mice were treated with control and TGFβR kinase inhibitor (75 mg/kg) daily for 14 days. The kidney sections were stained with Masson's Trichrome to visualize improvement in fibrosis.